new technique

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>>"We have developed a novel method for extraction of relatively long DNA
fragments from fossilized bone and fresh material for subsequent analysis
using PCR and standard sequencing techniques.  The advantage of this method
is that it selects for unmodified, undamaged, and chemically unaltered DNA
fragments greater than 300 base pairs in length.  Currently available
techniques produce only small fragments which can result in chimeric
formation of mosaic sequences after PCR.  True sequence divergence cannot be
obtained from these mosaic sequences.  Our method does not use conventional
extraction procedures such as mechanically grinding bone in the presence of
phenol, chloroform extractions, alcohol precipitation, or other harsh
chemical treatments that may damage already compromised DNA.  Our method
also includes a treatment that greatly reduces the chance of contaminant DNA.

Potential uses of this technology in forensic medicine and paleontology include:

1. Production of intact genes from extinct organisms
2. Identification of extinct species for which there are only small portions
of a skeleton remaining, or for which     there is only a small bone fragment
3. Phylogenetic classification of extinct animals and plants
4. Rapid recovery of intact DNA from bones or other material.

If you represent, or are in a position to forward this non-confidential
abstract to parties interested in commercial rights to this potentially
patentable process, or are able to fund research to further develope this
technique, with proprietary interests in mind, please contact:

Chip Pretzman
Dept. of Molecular Genetics
The Ohio State University
484 W. 12th AVe.
Columbus, OH  43210

614-292-4570
fax 614-292-4466
home 614-848-5056
internet cpretzma@magnus.acs.ohio-state.edu
CIS 76016,2272